Regulatory

Part:BBa_K1734001

Designed by: Eduardo Ramírez Montiel   Group: iGEM15_Tec-Monterrey   (2015-09-17)

OpIE2 promoter

The immediate-early 2 promoter from the multicapsid nucleopolyhedrosis virus Orgyia pseudotsugata (OpIE2) is a constitutive promoter. Usually is used in nonlytic gene expression systems [1], but can be used with the baculovirus expression vector system (BEVS) methodology as well.

Tec_Monterrey_Conf_Opie2.jpg

This part has been confirmed by restriction analysis of the pSB1C3 vector


Reference:

[1]Kempf, J., Snook, L. A., Vonesch, J.-L., Dahms, T. E. S., Pattus, F., & Massotte, D. (2002). Expression of the human μ opioid receptor in a stable Sf9 cell line. Journal of Biotechnology, 95(2), 181-187. doi:http://dx.doi.org/10.1016/S0168-1656(02)00008-1


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 290
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None